CloneMe! beta

Stage 1: What are we cloning today?

Option 1: annotated ORF

lpg (CGC annotation)     OR     NT04LP (TIGR annotation)
Number of bases upstream:     and downstream:

Option 2: your own coordinates

Start:     AND     End:     Reveresed?


Stage 2: What are we cloning with?

EcoRI    BamHI    PstI    XbaI    SalI

You may enter a restriction site of your own*:
Or otherwise choose some restriction enzymes out of this list: (Hold the 'Ctrl' for multiple selection.)

Mark this if you wish to receive a list of all single cutters.


* Ambiguous nucleotide codes of the IUPAC-IUB commission are supported:
(Upper and lower case are considered equivalent.)
Symbol Nucleotide(s) Description
A A Adenosine
C C Cytidine
G G Guanosine
T T Thymidine
R = A or G puRines
Y = C or T pYrimidines
W = A or T Weak hydrogen bonding
S = G or C Strong hydrogen bonding
M = A or C aMino group at common position
K = G or T Keto group at common position
H = A, C or T not G
B = G, C or T not A
V = G, A, C not T
D = G, A or T not C
N = G, A, C or T aNy